SF2 Antibody / SRSF1

SF2, also known as Serine/arginine-rich splicing factor 1 (SRSF1), is a protein that in humans is encoded by the SFRS1 gene. This gene encodes a member of the arginine/serine-rich splicing factor protein family. There is a pseudogene of this gene on chromosome 13. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. ASF/SF2 is necessary for all splicing reactions to occur, and influences splice site selection in a concentration-dependent manner, resulting in alternative splicing.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q07955

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids VIRGPAGNNDCRIYVGNLPPDIRTKDIE of human SF2 were used as the immunogen for the SF2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the SF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SF2 antibody is available for research use only.

Storage Conditions

After reconstitution, the SF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SF2 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human placental tissue with SF2 antibody. HIER: Boil the paraffin sections in pH8 EDTA for 20 minutes and allow to cool prior to staining.