Caspase-7 Antibody

Caspase-7, Apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. It is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD) -dependent pathway.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

P55210

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Amino acids 117-198 of human Caspase-7 were used as the immunogen for the Caspase-7 antibody. This sequence is from the large subunit.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trelahose

Reconstitution

After reconstitution, the Caspase-7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Caspase-7 antibody is available for research use only.

Storage Conditions

After reconstitution, the Caspase-7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Caspase-7 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) human A549, 2) rat spleen and 3) rat lung lysate using Caspase-7 antibody. Expected/observed molecular weight ~34 kDa (full length) .