Fos B Antibody

FOSB, FBJ murine osteosarcoma viral oncogene homolog B, is a protein that, in humans, is encoded by the FOSB gene. FOSB is a member of Fos gene family which consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. The FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. The FOSB gene is mapped to 19q13.32. Delta FOSB is a truncated splice variant of FOSB. Delta FosB has been implicated in the development of drug addiction and control of the reward system in the brain, and is linked to changes in a number of other gene products such as CREB and sirtuins. Delta FosB also regulates the commitment of mesenchymal precursor cells to the adipocyte or osteoblast lineage.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P53539

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids LPFQTSQDAPPNLTASLFTHSEVQVLGD of human Fos B were used as the immunogen for the Fos B antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Fos B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Fos B antibody is available for research use only.

Storage Conditions

After reconstitution, the Fos B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Fos B antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC testing of FFPE human pancreatic cancer tissue with Fos B antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.