MMP9 Antibody

Matrix metallopeptidase 9, or 92 kDa type IV collagenase, is part of a family of proteins involved in the breakdown of extracellular matrix in normal physiological processes. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP9 degrades type IV and V collagens.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (mouse protein tested) ; request BSA-free format for coating

UniProt

P41245

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids KALLFSKGRVWRFDLKSQKVDPQSVIRVDKEF of mouse MMP9 were used as the immunogen for the MMP9 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA (protein)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the MMP9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MMP9 antibody is available for research use only.

Storage Conditions

After reconstitution, the MMP9 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MMP9 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear, secreted

Image Legend

Western blot testing of 1) rat NRK, 2) mouse ANA-1 and 3) mouse HEPA cell lysate with MMP9 antibody. Predicted molecular weight: 92/67-80 kDa (precursor/mature forms), observed here at ~78 kDa.