STAT3 Antibody

The transcription factor, signal transducer and activator of transcription-3 (STAT-3) is the most pleiotropic member of the signal transducer and activator of transcription (STAT) family of transcription factors and mediates pivotal responses for the cytokine family. The mouse STAT3 gene contains 24 exons and spans 30 kb. The translation initiation codon is in exon 2, and the stop codon is in exon 24. STAT3 is mapped to 17q21. It contributes to various physiological processes. Hepatic STAT-3 signaling is thus essential for normal glucose homeostasis and may provide new therapeutic targets for diabetes mellitus.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug antibody/500ug lysate, Flow cytometry: 1-3ug/million cells

UniProt

P40763

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids EKQQMLEQHLQDVRKRVQDLEQKMKVVEN of human STAT3 were used as the immunogen for the STAT3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, IP, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the STAT3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This STAT3 antibody is available for research use only.

Storage Conditions

After reconstitution, the STAT3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the STAT3 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of 1) human HEL, 2) human A549, 3) rat lung, 4) rat heart, 5) mouse lung and 6) mouse heart tissue lysate with STAT3 antibody. Expected molecular weight: ~92 kDa (alpha) and ~83 kDa (beta) .