Cofilin Antibody

Cofilin, a member of the ADF/cofilin family, is actually a protein with 70% sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

P23528

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids DAIKKKLTGIKHELQANCYEEVKDRCTLAEK of human Cofilin were used as the immunogen for the Cofilin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Cofilin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cofilin antibody is available for research use only.

Storage Conditions

After reconstitution, the Cofilin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Cofilin antibody should be determined by the researcher.

Location

Cytoplasmic, membrane

Image Legend

Immunofluorescent staining of FFPE human MCF7 cells with Cofilin antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.