COMT Antibody

Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL

UniProt

P21964

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 52-271 of human COMT were used as the immunogen for the COMT antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the COMT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This COMT antibody is available for research use only.

Storage Conditions

After reconstitution, the COMT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the COMT antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) rat brain, 2) rat liver, 3) rat kidney, 4) mouse brain, 5) Jurkat, 6) CEM and 7) HeLa lysate with COMT antibody. Predicted molecular weight: ~30/25 kDa (isoforms 1/2) .