RAB5 Antibody

RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP) . RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

P20339

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN of human RAB5A were used as the immunogen for the RAB5 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the RAB5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This RAB5 antibody is available for research use only.

Storage Conditions

After reconstitution, the RAB5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the RAB5 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) rat brain, 2) mouse brain, and human 3) HeLa, 4) SW620, 5) 293 and 6) Jurkat lysate with RAB5 antibody. Expected/observed molecular weight ~25 kDa.