PU.1 Antibody / SPI1

Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (FFPE) : 0.5-1 µg/mL

UniProt

P17947

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 18-196 of human PU.1/SPI1 were used as the immunogen for the PU.1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the PU.1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PU.1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PU.1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PU.1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC testing of FFPE human tonsil with PU.1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.