ARSA Antibody

Arylsulfatase A (ARSA) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate intocerebroside and sulfate. In humans, arylsulfatase A is encoded by the ARSA gene. ARSA is mapped to 22q13.33. The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, FACS: 1-3ug/10^6 cells, IF/ICC (FFPE) : 2-4 µg/mL

UniProt

P15289

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids QALKQLQLLKAQLDAAVTFGPSQVARGED of human ARSA were used as the immunogen for the ARSA antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF/ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the ARSA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ARSA antibody is available for research use only.

Storage Conditions

After reconstitution, the ARSA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ARSA antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) rat testis, 2) rat pancreas, 3) rat skeletal muscle, 4) mouse kidney and 5) human MCF7 lysate with ARSA antibody. Expected molecular weight: 53-63 kDa.