Ribonuclease Inhibitor Antibody / RNH1

Ribonuclease inhibitor is an enzyme that in humans is encoded by the RNH1 gene. Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular Rnases. In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin. Ribonuclease inhibitor binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P13489

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Amino acids SLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDD of human RNH1 were used as the immunogen for the Ribonuclease Inhibitor antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Ribonuclease inhibitor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Ribonuclease Inhibitor antibody is available for research use only.

Storage Conditions

After reconstitution, the Ribonuclease Inhibitor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Ribonuclease Inhibitor antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human intestinal cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.