Annexin A3 Antibody

Annexin A3 is a protein that in humans is encoded by the Annexin A3 gene. The Annexin A3 gene contains 13 exons and spans 58 kb of genomic DNA. The Annexin A3 gene is mapped to 4q21. It is abnormally expressed in fetuses of both IVF and ICSI, which may contribute to the increase risk of birth defects in these ART. This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phospholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, ICC (FFPE) : 0.5-1 µg/mL

UniProt

P12429

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids RKALLTLADGRRDESLKVDEHLAKQDAQILYK of human Annexin A3 were used as the immunogen for the Annexin A3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Annexin A3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Annexin A3 antibody is available for research use only.

Storage Conditions

After reconstitution, the Annexin A3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Annexin A3 antibody should be determined by the researcher.

Location

Cytoplasmic, membranous

Image Legend

Western blot testing of 1) rat spleen, 2) rat stomach, 3) human HeLa, 4) 22RV1, 5) A549, 6) MCF7 and 7) SW620 antibody with Annexin A3 antibody. Predicted/observed molecular weight ~36 kDa.