Cdk4 Antibody

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. And CDK4 expression and activity are required for cytokine responsiveness in T cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL

UniProt

P11802

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 201-303 of human Cdk4 were used as the immunogen for the Cdk4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Cdk4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cdk4 antibody is available for research use only.

Storage Conditions

After reconstitution, the Cdk4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Cdk4 antibody should be determined by the researcher.

Location

Nuclear and cytoplasmic

Image Legend

Western blot testing of 1) human HeLa, 2) human K562, 3) rat PC-12, 4) mouse NIH 3T3 and 5) mouse RAW264.7 cell lysate with Cdk4 antibody. Predicted molecular weight ~34 kDa.