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Lipocalin 2 Antibody (NGAL, LCN2)

Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (mouse protein tested) ; request BSA-free format for coating

UniProt

P11672

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids 21-200 of mouse Lipocalin 2 were used as the immunogen for the Lipocalin 2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F, ELISA (protein)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the Lipocalin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Lipocalin 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Lipocalin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Lipocalin 2 antibody should be determined by the researcher.

Location

Cytoplasmic, secreted

Image Legend

Western blot testing of 1) rat spleen and 2) rat lung lysate with Lipocalin 2 antibody. Predicted/observed molecular weight: 22-25 kDa.

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