CLU Antibody / Clusterin / Apolipoprotein J / APO-J

Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. In humans, clusterin is encoded by the CLU gene. This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI) . Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested) ; request BSA-free format for coating

UniProt

P10909

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids 228-449 of human Clusterin/Apolipoprotein J were used as the immunogen for the CLU antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA (protein)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the CLU antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CLU antibody is available for research use only.

Storage Conditions

After reconstitution, the CLU antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CLU antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) SKOV, 2) U87 and 3) PANC cell lysate with CLU antibody. Predicted molecular weight: 75-80 kDa (heterodimer precursor), 36-39 kDa (alpha subunit), 34-36 kDa (beta subunit) .