Csf-1 Antibody

Macrophage Colony Stimulating Factor 1, also called MCSF or CSF1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival ofmonocytes, macrophages, and bone marrow progenitor cells. MCSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of MCSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or MCSFR encoded by the c-fms proto-oncogene), MCSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (FFPE) : 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (mouse protein tested) ; request BSA-free format for coating

UniProt

P07141

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids 33-262 of mouse Colony stimulating factor 1 were used as the immunogen for the Csf-1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, ELISA (protein)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Csf-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Csf-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Csf-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Csf-1 antibody should be determined by the researcher.

Location

Cytoplasmic, membrane

Image Legend

IHC testing of FFPE mouse spleen with Csf-1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.