CDK1 Antibody / CDC2

CDC2, Cell Division Cycle 2, is also known as CDK1 (Cyclin-dependent Kinase 1) . CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence/Immunocytochemistry: 2-4 µg/mL, Flow cytometry: 1-3ug/10^6 cells

UniProt

P06493

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 66-297 of human CDK1/CDC2 were used as the immunogen for the CDK1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF/ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the CDK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CDK1 antibody is available for research use only.

Storage Conditions

After reconstitution, the CDK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CDK1 antibody should be determined by the researcher.

Location

Nuclear and cytoplasmic

Image Legend

Western blot testing of 1) rat thymus, 2) rat spleen, 3) human MCF7, 4) HeLa, 5) Jurkat, and 6) mouse NIH3T3 lysate with CDK1 antibody. Predicted/observed molecular weight: ~33 kDa.