PRDX1 Antibody / Peroxiredoxin 1

Peroxiredoxin 1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes which reduce hydrogen peroxide and alkyl hydroperoxides.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 2 µg/mL, Flow cytometry: 1-3ug/million cells

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the middle region of human Peroxiredoxin 1 (MNIPLVSDPKRTIAQDYGVLKADEGISFR) was used as the immunogen for this PRDX1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

The PRDX1 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Peroxiredoxin 1 antibody is available for research use only.

Storage Conditions

The PRDX1 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the PRDX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescent staining of FFPE human U-2 OS cells with PRDX1 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.