LYRIC Antibody / Metadherin / AEG-1

MTDH (Metadherin), also known as LYRIC or astrocyte elevated gene-1 protein (AEG-1), is a protein that in humans is encoded by the MTDH gene. It is involved in HIF-1 alpha mediated angiogenesis, interacts with SND1 and is involved in RNA-induced silencing complex (RISC), and plays a very important role in RISC and miRNA functions. LYRIC induces an oncogene called Late SV40 factor (LSF/TFCP2) which is involved in thymidylate synthase (TS) induction and DNA biosynthesis synthesis. Late SV40 factor enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9) . LYRIC acts as an oncogene in melanoma, malignant glioma, breast cancer and hepatocellular carcinoma. It is highly expressed in these cancers and helps in progression and development of these cancers. It is induced by c-Myc oncogene and plays very important role in anchorage independent growth of cancer cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, ICC (FFPE) : 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 101-270) was used as the immunogen for this LYRIC antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the LYRIC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This LYRIC antibody is available for research use only.

Storage Conditions

After reconstitution, the LYRIC antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the LYRIC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

Western blot testing of human 1) U-87 MG, 2) HeLa, 3) MDA-MB-453, 4) PC-3, 5) A431, 6) Caco-2 and 7) HepG2 cell lysate with LYRIC antibody. Expected molecular weight: 70-80 kDa.