HSP60 Antibody / HSPD1
NFKBTBETTLR2
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 2-3 µg/mL, Flow cytometry: 1-3ug/10^6 cells
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 260-496) was used as the immunogen for this HSP60 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the HSP60 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HSP60 antibody is available for research use only.
Storage Conditions
After reconstitution, the HSP60 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the HSP60 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of HSP60 antibody and Lane 1: rat kidney; 2: mouse kidney; 3: (m) HEPA; 4: (r) NRK; 5: (r) PC12; 6: human SW620; 7: (h) A549; 8: (h) A431; 9: (h) HeLa lysate. Predicted/observed molecular weight: ~60 kDa.
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