Cytochrome C Antibody

CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 1-2 µg/mL

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 2-105) was used as the immunogen for this Cytochrome C antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

Store the Cytochrome C antibody at 4oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cytochrome C antibody is available for research use only.

Storage Conditions

Store the Cytochrome C antibody at 4°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the Cytochrome C antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of Cytochrome C antibody and Lane 1: rat brain; 2: mouse brain; 3: (r) heart; 4: (m) heart; 5: human U87; 6: (m) Neuro-2a; 7: (h) HeLa; 8: (h) Jurkat; 9: (h) placenta lysate. Predicted molecular weight: 12~14 kDa.