Rab3A Antibody

Ras-related protein Rab-3A is a protein that in humans is encoded by the RAB3A gene. Synapsin I stimulated the Rab3A cycle by increasing GTP binding, GTPase activity, and Rab3A recruitment to the synaptic vesicle membrane. Conversely, Rab3A inhibited Synapsin I binding to actin and Synapsin I-induced synaptic vesicle clustering. It has been found that the protein is necessary for both forms of synaptic plasticity, and it is also involved in calcium exocytosis in neurons. In addition to that, Rab3A has been found to regulate a late step in synaptic vesicle fusion.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 192-220 (DTADPAVTGAKQGPQLSDQQVPPHQDCAC-human) were used as the immunogen for this Rab3A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Rab3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Rab3A antibody is available for research use only.

Storage Conditions

After reconstitution, the Rab3A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Rab3A antibody may be required due to differences in protocols and secondary/substrate sensitivity.