Oct-1 Antibody / POU2F1

POU domain class 2 transcription factor 1, also known as OTF1 (Octamer-binding transcription factor 1) or Oct-1 (Octamer-binding protein 1), is a protein that in humans is encoded by the POU2F1 gene. The transcription factor was among the first identified members of the POU transcription factor family. It has been found that the Oct-1 dimer formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1 , whereas dimers formed on the consensus MORE (more PORE) (ATGCATATGCAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) failed to interact. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

P14859

Host

Rabbit

Reactivity

Human

Immunogen

Human partial recombinant protein (AA 11-240) was used as the immunogen for this Oct-1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Oct-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Oct-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Oct-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Oct-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Oct-1 antibody and Lane 1: rat liver; and human samples 2: placenta; 3: Jurkat; 4: HeLa; 5: A549; 6: SMMC-7721. Predicted molecular weight ~89 kDa.