Profilin-1 Antibody

Profilin-1 is a protein that in humans is encoded by the PFN1 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. At high concentrations, Profilin-1 prevents the polymerization of actin, whereas it enhances it at low concentrations. This gene is a 140-amino acid protein and major growth regulator of filamentous (F) -actin through its binding of monomeric (G) -actin. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human PFN1 (KEGVHGGLINKKCYEMASHLRRSQY) was used as the immunogen for this Profilin-1 antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Profilin-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Profilin-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Profilin-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Profilin-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Profilin-1 antibody and Lane 1: rat testis; 2: mouse testis; 3: human placenta; 4: (h) PANC; 5: (h) SW620 lysate. Expected molecular weight ~15 kDa.