PDGF-B Antibody

Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. PDGF-B can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGF-B plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGF-A.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 82-190) was used as the immunogen for this PDGF-B antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the PDGF-B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PDGF-B antibody is available for research use only.

Storage Conditions

After reconstitution, the PDGF-B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the PDGF-B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of PDGF-B antibody and Lane 1: rat heart; 2: (r) brain; 3: mouse heart; 4: human HeLa lysate Predicted size: ~15 kDa monomer and ~32 kDa dimer.