DJ-1 Antibody / PARK7

Parkinson disease 7, also known as Protein deglycase DJ-1, is a protein which in humans is encoded by the PARK7 gene. PARK7 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It is also involved in tumorigenesis and in maintaining mitochondrial homeostasis. DJ-1 may function as a redox-sensitive chaperone, as a sensor foroxidative stress, and it apparently protects neurons against oxidative stress and cell death. It has been found that DJ-1 mutations that impair transcriptional coactivator function can render dopaminergic neurons vulnerable to apoptosis and may contribute to the pathogenesis of Parkinson disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 2-189) was used as the immunogen for this DJ-1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, IP

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the DJ-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

DJ-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the DJ-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the DJ-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic & Nuclear

Image Legend

Immunofluorescent staining of FFPE human SK-O-V3 cells with DJ-1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.