PAPP-A Antibody / Pappalysin-1

Pappalysin-1, also known as DIPLA1, is a protein that in humans is encoded by the PAPPA gene. PAPP-A is found in the ovarian follicles, follicular fluid, luteal cells, and fallopian tubes of nonpregnant women and in the seminal vesicles and seminal fluid of males. This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs) . It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. It has been found that circulating PAPP-A is a disulfide-bridged complex with proMBP in which the subunits of the constituents are present in a 1:1 molar ratio.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

Q13219

Host

Rabbit

Reactivity

Human

Immunogen

Human partial recombinant protein (AA 95-388) was used as the immunogen for this PAPP-A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the PAPP-A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PAPP-A antibody is available for research use only.

Storage Conditions

After reconstitution, the PAPP-A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the PAPP-A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of PAPP-A antibody and human samples 1: placenta; 2: HT1080; 3: SKOV; 4: 22RV1; 5: SW620; 6: MM231 lysate. Predicted/observed size ~180KD