NUMB Antibody

MDM2Notch

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P49757

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Amino acids 194-220 (SFRVTTATEQAEREEIMKQMQDAKKAE-human) were used as the immunogen for this NUMB antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Numb antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NUMB antibody is available for research use only.

Storage Conditions

After reconstitution, the NUMB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the NUMB antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasm, cell membrane

Image Legend

IHC staining of FFPE human liver cancer tissue with NUMB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.