NUMB Antibody
MDM2Notch
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P49757
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Amino acids 194-220 (SFRVTTATEQAEREEIMKQMQDAKKAE-human) were used as the immunogen for this NUMB antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Numb antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NUMB antibody is available for research use only.
Storage Conditions
After reconstitution, the NUMB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the NUMB antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasm, cell membrane
Image Legend
IHC staining of FFPE human liver cancer tissue with NUMB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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