Nod1 Antibody

Nucleotide-binding oligomerization domain-containing protein 1, also known as CARD4, is a protein receptor that in humans is encoded by the NOD1 gene. It is a member of NOD-like receptor protein family and is a close relative of Nod2. It recognizes bacterial molecules and stimulates an immune reaction. Nod1 contains a caspase recruitment domain (CARD) . This gene is an intracellular pattern recognition receptor, which is similar in structure to resistant proteins of plants, and mediates innate and acquired immunity by recognizing bacterial molecules containing D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety. Additionally, it has been shown that Nod1 can sense cytosolic microbial products by monitoring the activation state of small Rho GTPases.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, ICC (FFPE) : 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Human partial recombinant protein (AA 1-160) was used as the immunogen for this Nod1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the Nod1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Nod1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Nod1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Nod1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.