NKX2.5 Antibody

Homeobox protein Nkx-2.5, also known as NKX2E or CSX, is a protein that in humans is encoded by the NKX2-5 gene. It is mapped to 5q35.1. Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. Nkx2-5 and Tbx5 directly bound to the promoter of the gene encoding cardiac-specific natriuretic peptide precursor type A (NPPA) in tandem, and both transcription factors showed synergistic activation. Nkx2-5 is essential in cardiac development, and mutations in the protein cause various congenital heart malformations.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

An amino acid sequence from the middle region of human Homeobox protein Nkx-2.5 (AKDPRAEKKELCALQKAVELEKTEADNAER) was used as the immunogen for this NKX2.5 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Nkx2-5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NKX2.5 antibody is available for research use only.

Storage Conditions

After reconstitution, the NKX2.5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the NKX2.5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of NKX2.5 antibody and Lane 1: mouse spleen; 2: mouse heart; 3: human HeLa lysate. Predicted molecular weight ~35 kDa, routinely observed at 35~45kDa.