ABCB4 Antibody / MDR3

Adenosine triphosphate-binding cassette subfamily B, member 4, also called MDR3, is a protein that in humans is encoded by the ABCB4 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABCB4 mutations represent a genetic factor involved in this peculiar form of cholesterol gallstone disease in adults.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL, FACS: 1-3ug/million cells, Western blot: 0.5-1 µg/mL

UniProt

P21439

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 601-720) was used as the immunogen for this ABCB4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

FACS, IHC-P, WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

The lyophilized ABCB4 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

Limitations

This ABCB4 antibody is available for research use only.

Storage Conditions

The lyophilized ABCB4 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Variations in secondary/substrate sensitivities and test protocols may require the ABCB4 antibody to be titrated for optimal performance.

Location

Cell membrane, cytoplasm

Image Legend

IHC staining of FFPE human liver cancer with ABCB4 antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.