MIF Antibody / Macrophage migration inhibitory factor
Macrophage migration inhibitory factor (MMIF), also known as GIF, is a protein that in humans is encoded by the MIF gene. It is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. The gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. MIF plays a critical role in inflammatory diseases and atherogenesis. It is also involved in cell-mediated immunity and immunoregulation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P14174
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 2-115) was used as the immunogen for this MIF antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
The lyophilized MIF antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.
Limitations
This MIF antibody is available for research use only.
Storage Conditions
The lyophilized MIF antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Variations in secondary/substrate sensitivities and test protocols may require the MIF antibody to be titrated for optimal performance.
Location
Cytoplasmic, secreted
Image Legend
Documents
Frequently Asked Questions
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