Von Willebrand Factor Antibody

Von Willebrand Factor is a blood glycoprotein involved in hemostasis. The VWF gene encodes a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. vWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. Deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Mouse partial recombinant protein (AA 1304-1452) was used as the immunogen for this von Willebrand Factor antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

The von Willebrand Factor antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

Limitations

This von Willebrand Factor antibody is available for research use only.

Storage Conditions

The von Willebrand Factor antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Variations in secondary/substrate sensitivities and test protocols may require the von Willebrand Factor antibody to be titrated for optimal performance.