MSK1 Antibody / RPS6KA5
Ribosomal protein S6 kinase alpha-5, also known as MSK1, is an enzyme that in humans is encoded by the RPS6KA5 gene. It belongs to the AGC family of kinases. MSK1 gene is mapped to chromosome 14q31-q32. Northern blot analysis indicated that MSK1 was expressed as a 4-kb mRNA in all tissues tested, with the highest levels of expression in brain, muscle, and placenta. MSK1 was activated in vitro and in vivo by either ERK or SAPK2 proteins. It has been found that MSK1, rather than MAPKAP-K1 or MAPKAP-K2/K3, mediates activation of the cAMP response element-binding protein and activating transcription factor-1 by either growth factors or stress signals.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 540-665) was used as the immunogen for this MSK1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC, IF
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
Limitations
This MSK1 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Location
Nuclear + cytoplasmic
Image Legend
IHC staining of FFPE human placental tissue with MSK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Frequently Asked Questions
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