Menin Antibody

The MEN1 gene encodes Menin, a nuclear scaffold protein that regulates gene transcription by coordinating chromatin remodeling. It is mapped to 11q13.1. MEN1 is considered to act as a tumor suppressor gene. It has been found that that MEN1 inactivation by antisense RNA antagonizes transforming growth factor-beta-mediated cell growth inhibition. Overexpression in an inducible cell culture system downregulated the proximal promoter. In vitro studies have shown that the protein is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD. Additionally, MEN1 was essential to maintain MLL-associated myeloid transformation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Human partial recombinant protein (AA 301-615) was used as the immunogen for this Menin antibody. Human MEN1 shares 93% and 94% amino acid sequence identity with mouse and rat, respectively.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Menin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Menin antibody is available for research use only.

Storage Conditions

After reconstitution, the Menin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Menin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Menin antibody and Lane 1: human HeLa; 2: (h) 293T; 3: (h) SMMC-7721; 4: mouse HEPA1-6; 5: (h) COLO320. Predicted molecular weight ~68 kDa.