MAD1 Antibody

Mitotic spindle assembly checkpoint protein MAD1 is a protein that in humans is encoded by the MAD1L1 gene. It is mapped to 7p22.3. MAD1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. It can function as a homodimer. It localizes to the centrosome during metaphase and to the spindle midzone and the midbody during anaphase and telophase. MAD1 may also play a role in cell cycle control and tumor suppression.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunocytochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL

UniProt

Q9Y6D9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 362-632) was used as the immunogen for this MAD1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the MAD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MAD1 antibody is available for research use only.

Storage Conditions

After reconstitution, the MAD1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the MAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) rat brain, 2) rat kidney, 3) rat lung, 4) rat liver, 5) mouse brain, 6) mouse kidney, 7) mouse lung, 8) mouse liver and 9) mouse NIH3T3 lysate. Predicted/observed molecular weight ~83 kDa.