HIF-1 alpha Antibody
Hypoxia-inducible factor 1a (HIF-1 alpha) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. It is crucial for the cellular response to hypoxia and plays a pivotal role in cellular adaptation to changes in oxygen availability.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 703-732 (EEELNPKILALQNAQRKRKMEHDGSLFQAV-human) were used as the immunogen for this HIF-1 alpha antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
The lyophilized HIF-1-alpha antibody can be stored at 4oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HIF-1 alpha antibody is available for research use only.
Storage Conditions
The lyophilized HIF-1 alpha antibody can be stored at 4°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear, possible cytoplasmic
Image Legend
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Frequently Asked Questions
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