HIF-1 alpha Antibody

Hypoxia-inducible factor 1a (HIF-1 alpha) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. It is crucial for the cellular response to hypoxia and plays a pivotal role in cellular adaptation to changes in oxygen availability.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 703-732 (EEELNPKILALQNAQRKRKMEHDGSLFQAV-human) were used as the immunogen for this HIF-1 alpha antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

The lyophilized HIF-1-alpha antibody can be stored at 4oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HIF-1 alpha antibody is available for research use only.

Storage Conditions

The lyophilized HIF-1 alpha antibody can be stored at 4°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, possible cytoplasmic

Image Legend

Western blot testing of HIF-1 alpha antibody and Lane 1: human HeLa; 2: human SHG-44; 3: mouse HEPA1-6; Routinely observed molecular weight: 100~120 kDa.