Fas Antibody / CD95

Fas (also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. FAS antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor (TNF) receptors and the low-affinity nerve growth factor receptor (NGFR) and it is mapped to 10q24.1. The FAS/FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human

Immunogen

Human partial recombinant protein (AA 26-173) was used as the immunogen for this Fas antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Fas antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Fas antibody is available for research use only.

Storage Conditions

After reconstitution, the Fas antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Fas antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, membranous

Image Legend

Western blot testing of Fas antibody and Lane 1: HeLa; 2: Jurkat; 3: A549; 4: SMMC-7721; 5: K562 lysate. Predicted molecular weight: ~38 kDa (unmodified), 40-55 kDa (glycosylated) .