Heme Oxygenase 2 Antibody / HO-2 / HMOX2

Heme oxygenase 2, also known as HO-2, is an enzyme that in humans is encoded by the HMOX2 gene. It is mapped to 16p13.3. Heme oxygenases cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 2-316) was used as the immunogen for this Heme Oxygenase 2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Heme Oxygenase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Heme Oxygenase 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Heme Oxygenase 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Heme Oxygenase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Heme Oxygenase 2 antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.