C5 Antibody / Complement 5A
C5a is a protein fragment released from complement component C5. The protein plays an important role in inflammatory and cell killing processes. C5 is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. C5a plays a key role in increasing migration and adherence of neutrophils and monocytes to vessel walls. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
Host
Rabbit
Reactivity
Rat
Immunogen
Rat partial recombinant protein (AA 1-77) was used as the immunogen for this C5 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
The C5 antibody can be stored at 4oC to -20oC. After reconsititution, aliquot and store at -20oC. Avoid repeated freeze/thaws.
Limitations
This C5 antibody is available for research use only.
Storage Conditions
The C5 antibody can be stored at 4°C to -20°C. After reconsititution, aliquot and store at -20°C. Avoid repeated freeze/thaws.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Titration of the C5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of C5 antibody and rat liver lysate; Predicted molecular weight: ~115 kDa (C5 alpha chain) .
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