Furin Antibody

Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. Furin is thought to play a role in tumor progression. It is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression in T-cells is required for maintenance of peripheral immune tolerance.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human

Immunogen

Human partial recombinant protein (AA 591-794) was used as the immunogen for this Furin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Furin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Furin antibody is available for research use only.

Storage Conditions

After reconstitution, the Furin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Furin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Furin antibody and Lane 1: HeLa; 2: MCF-7; 3: COLO320; 4: SW620. Observed molecular weight 90~110 kDa (precusor/mature may be glycosylated) .