Decorin Antibody

Decorin is a protein that in humans is encoded by the DCN gene. This gene is mapped to 12q21.3. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS) . Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to Biglycan protein. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. And it also may play a role in epithelial/mesenchymal interactions during organ development and shaping. Decorin has been shown to have anti-tumorigenic properties in an experimental murine tumor model and is capable of suppressing the growth of various tumor cell lines.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 31-359) was used as the immunogen for this Decorin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Decorin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Decorin antibody is available for research use only.

Storage Conditions

After reconstitution, the Decorin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Decorin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC-P: Decorin antibody testing of human breast cancer tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.