BRAF Antibody (B Raf)
BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P15056
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 38-230) was used as the immunogen for this BRAF antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
The lyophilized BRAF antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This BRAF antibody is available for research use only.
Storage Conditions
The lyophilized BRAF antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasm, nucleus, plasma membrane
Image Legend
IHC staining of FFPE human placental tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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