P63 Antibody / Tumor protein 63

Tumor protein p63 (TP63), also known as TP73L is a protein that in humans is encoded by the TP63 gene. It is a member of the p53 family of transcription factors. This gene encodes for two main isoforms by alternative promoters (TAp63 and dNp63) . TP63 has been mostly restricted to its apoptotic function and more recently as the guardian of oocyte integrity. It has been found that the combined loss of TP63 and p73 results in the failure of cells containing functional p53 to undergo apoptosis in response to DNA damage. It is an essential regulator of stem cell maintenance in stratified epithelial tissues. TP63 is also critical for maintaining the progenitor-cell populations that are necessary to sustain epithelial development and morphogenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9H3D4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 311-680) was used as the immunogen for this p63 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the p63 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This p63 antibody is available for research use only.

Storage Conditions

After reconstitution, the p63 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the p63 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescent staining of FFPE human prostate hyperplasia tissue with p63 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.