NHE1 Antibody / SLC9A1

NHE1, also known as SLC9A1, is an isoform of sodium-hydrogen antiporter that in humans is encoded by the SLC9A1 gene. It is mapped to 1p36.11. NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others. The plasma membrane ion exchanger NHE1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. And a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is a plausible candidate gene for human essential hypertension.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human recombinant partial protein (AA 543-815) was used as the immunogen for this NHE1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the NHE1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NHE1 antibody is available for research use only.

Storage Conditions

After reconstitution, the NHE1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the NHE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.