MAC-1 Antibody / CD11b / ITGAM

Macrophage-1 alpha (MAC-1) or CD11b are glycoproteins encoded by the ITGAM gene. It is mapped to 16p11.2. MAC-1 has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Additionally, it likely recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 17-382) was used as the immunogen for this MAC-1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the MAC-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MAC-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the MAC-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the MAC-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. This antibody has shown cross-reactivity with CD11c by ELISA.