Bub1 Antibody

Budding uninhibited by benzimidazoles 1, also known as Mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It was first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of Bub1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. Bub1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

O43683

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Human partial recombinant protein (AA 731-1085) was used as the immunogen for this Bub1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Bub1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Bub1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Bub1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Bub1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) K562, 2) HeLa, 3) 293T, 4) A431, 5) Caco-2, 6) PC-3, 7) MCF7, 8) U-2 OS, 9) rat testis and 10) mouse testis lysate with Bub1 antibody at 0.5ug/ml. Predicted molecular weight ~122 kDa.