MCL1 Antibody

Myeloid cell leukemia 1 is a protein that in humans is encoded the MCL1 gene. It is a potent multidomain antiapoptotic protein of the BCL2 family that heterodimerizes with other BCL2 family members to protect against apoptotic cell death. MCL1 as an attractive candidate for regulation of hematopoietic stem cell homeostasis that is highly expressed in hematopoietic stem cells and regulated by growth factor signals. It is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors. During mitotic arrest, MCL1 protein levels decline markedly, through a posttranslational mechanism, potentiating cell death. Phosphorylation of the protein directs its interaction with the tumor suppressor protein FBW7, which is the substrate-binding component of a ubiquitin ligase complex.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 1-350) was used as the immunogen for this MCL1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the MCL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MCL1 antibody is available for research use only.

Storage Conditions

After reconstitution, the MCL1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the MCL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.